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FCS Viewer

FCS Viewer

chillerb

|
3 installs
| (0) | Free
Inspect flow cytometry data from FCS files in VS Code
Installation
Launch VS Code Quick Open (Ctrl+P), paste the following command, and press enter.
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vscode-fcs-viewer

Inspect flow cytometry data from FCS files in VS Code.

This extension was almost exclusively vibe-coded with claude-code. I don't guarantee for its correctness, but its fun to play around with.

FCS Viewer Plots

Features

  • FCS Parser: Open and inspect FCS files.
  • Plot Cards: Interactive Scatter Plots, Contour Plots, and Histograms cards thanks to React and plotly-js.
  • Compensation: Optionally apply the spillover compensation from the files $SPILLOVER metadata key.
  • Overview and table: All metadata, per-channel statistics, the spillover matrix, and a event table.
  • Dimensionality Reduction: Visualization of high-dimensional cytometry data via UMAP provided by umap-js.
  • Save and load workspaces: Save plot configurations as a workspace and come back to it later.

Commands

FCS Viewer provides 3 commands to manage workspaces:

Command Does
Open Workspace… Pick a saved workspace, or start a new empty one
Save Workspace… Name the current workspace's samples and plot cards
Discard Workspace… Delete a saved workspace; the FCS files are untouched

Right-clicking a .fcs file adds Open in FCS Viewer (always a new workspace) and, when one is already open, Add to FCS Viewer Workspace.

Settings

Setting Default Meaning
fcsViewer.defaultSampleSize 5000 Events plot cards render by default
fcsViewer.defaultCofactor 0 Arsinh cofactor; 0 picks from $CYT
fcsViewer.maxFileSizeMB 1024 Prompt before opening larger files
fcsViewer.maxSliceMB 16 Confirm before transferring more than this

Limitations

  • Gating: Gating is currently not supported.
  • Editing: The extension is currently read-only and never writes to your FCS files.
  • t-SNE: UMAP was preferred over t-SNE for efficiency.

Acknowledgements

  • Claude, duh
  • Flowkit by Scott White
  • umap-js and plotly-js
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